Show simple item record Bukosza, Eva Nora Kaucsár, Tamás Godó, Mária Lajtár, Enikő Tod, Pál Koncsos, Gábor Varga, Zoltán V Baranyai, Tamás Tu, Nguyen Minh Schachner, Helga Sőti, Csaba Ferdinandy, Péter Giricz, Zoltán Szénási, Gábor Hamar, Péter 2019-10-07T06:26:32Z 2019-10-07T06:26:32Z 2019
dc.identifier.citation journalVolume=20;journalIssueNumber=17;pagination=4266, pages: 17;journalTitle=INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES;journalAbbreviatedTitle=INT J MOL SCI;
dc.identifier.uri doi:10.3390/ijms20174266
dc.description.abstract Feeding rats with high-fat diet (HFD) with a single streptozotocin (STZ) injection induced obesity, slightly elevated fasting blood glucose and impaired glucose and insulin tolerance, and caused cardiac hypertrophy and mild diastolic dysfunction as published before by Koncsos et al. in 2016. Here we aimed to explore the renal consequences in the same groups of rats. Male Long-Evans rats were fed normal chow (CON; n = 9) or HFD containing 40% lard and were administered STZ at 20 mg/kg (i.p.) at week four (prediabetic rats, PRED, n = 9). At week 21 blood and urine samples were taken and kidney and liver samples were collected for histology, immunohistochemistry and for analysis of gene expression. HFD and STZ increased body weight and visceral adiposity and plasma leptin concentration. Despite hyperleptinemia, plasma C-reactive protein concentration decreased in PRED rats. Immunohistochemistry revealed elevated collagen IV protein expression in the glomeruli, and Lcn2 mRNA expression increased, while Il-1β mRNA expression decreased in both the renal cortex and medulla in PRED vs. CON rats. Kidney histology, urinary protein excretion, plasma creatinine, glomerular Feret diameter, desmin protein expression, and cortical and medullary mRNA expression of TGF-β1, Nrf2, and PPARγ were similar in CON and PRED rats. Reduced AMPKα phosphorylation of the autophagy regulator Akt was the first sign of liver damage, while plasma lipid and liver enzyme concentrations were similar. In conclusion, glomerular collagen deposition and increased lipocalin-2 expression were the early signs of kidney injury, while most biomarkers of inflammation, oxidative stress and fibrosis were negative in the kidneys of obese, prediabetic rats with mild heart and liver injury.
dc.relation.ispartof urn:issn:1661-6596
dc.title Glomerular Collagen Deposition and Lipocalin-2 Expression Are Early Signs of Renal Injury in Prediabetic Obese Rats
dc.type Journal Article 2019-09-09T08:50:02Z
dc.language.rfc3066 en
dc.rights.holder NULL
dc.identifier.mtmt 30789521
dc.identifier.pubmed 31480394
dc.contributor.department SE/AOK/I/Farmakológiai és Farmakoterápiás Intézet
dc.contributor.department SE/AOK/I/Kórélettani Intézet
dc.contributor.department SE/AOK/I/Orvosi Vegytani, Molekuláris Biológiai és Patobiokémiai Intézet
dc.contributor.institution Semmelweis Egyetem
dc.mtmt.swordnote Megosztott utolsó szerzőség.

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