dc.contributor.author | Szeder, Bálint | |
dc.contributor.author | Tárnoki-Zách, Júlia | |
dc.contributor.author | Lakatos, Dóra | |
dc.contributor.author | Vas, Virág | |
dc.contributor.author | Kudlik, Gyöngyi | |
dc.contributor.author | Merő, Balázs | |
dc.contributor.author | Koprivanacz, Kitti | |
dc.contributor.author | Bányai, László | |
dc.contributor.author | Hámori, Lilla | |
dc.contributor.author | Róna, Gergely | |
dc.contributor.author | Czirók, András | |
dc.contributor.author | Füredi, András | |
dc.contributor.author | Buday, László | |
dc.date.accessioned | 2019-12-04T13:13:11Z | |
dc.date.available | 2019-12-04T13:13:11Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | journalVolume=8;journalIssueNumber=11;pagination=E1343, pages: 18;journalTitle=CELLS;journalAbbreviatedTitle=CELLS; | |
dc.identifier.uri | http://repo.lib.semmelweis.hu//handle/123456789/8005 | |
dc.identifier.uri | doi:10.3390/cells8111343 | |
dc.description.abstract | Epithelial to mesenchymal transition (EMT) is a multipurpose process involved in wound healing, development, and certain pathological processes, such as metastasis formation. The Tks4 scaffold protein has been implicated in cancer progression; however, its role in oncogenesis is not well defined. In this study, the function of Tks4 was investigated in HCT116 colon cancer cells by knocking the protein out using the CRISPR/Cas9 system. Surprisingly, the absence of Tks4 induced significant changes in cell morphology, motility, adhesion and expression, and localization of E-cadherin, which are all considered as hallmarks of EMT. In agreement with these findings, the marked appearance of fibronectin, a marker of the mesenchymal phenotype, was also observed in Tks4-KO cells. Analysis of the expression of well-known EMT transcription factors revealed that Snail2 was strongly overexpressed in cells lacking Tks4. Tks4-KO cells showed increased motility and decreased cell-cell attachment. Collagen matrix invasion assays demonstrated the abundance of invasive solitary cells. Finally, the reintroduction of Tks4 protein in the Tks4-KO cells restored the expression levels of relevant key transcription factors, suggesting that the Tks4 scaffold protein has a specific and novel role in EMT regulation and cancer progression. | |
dc.relation.ispartof | urn:issn:2073-4409 | |
dc.title | Absence of the Tks4 Scaffold Protein Induces Epithelial-Mesenchymal Transition-Like Changes in Human Colon Cancer Cells | |
dc.type | Journal Article | |
dc.date.updated | 2019-11-25T12:50:07Z | |
dc.language.rfc3066 | en | |
dc.rights.holder | NULL | |
dc.identifier.mtmt | 30924768 | |
dc.identifier.pubmed | 31671862 | |
dc.contributor.department | SE/AOK/I/Orvosi Vegytani, Molekuláris Biológiai és Patobiokémiai Intézet | |
dc.contributor.institution | Semmelweis Egyetem |