dc.contributor.author |
Tod, Pál |
|
dc.contributor.author |
Bukosza, Eva Nora |
|
dc.contributor.author |
Róka, Beáta |
|
dc.contributor.author |
Kaucsár, Tamás |
|
dc.contributor.author |
Fintha, Attila |
|
dc.contributor.author |
Krenács, Tibor |
|
dc.contributor.author |
Szénási, Gábor |
|
dc.contributor.author |
Hamar, Péter |
|
dc.date.accessioned |
2020-08-28T11:19:48Z |
|
dc.date.available |
2020-08-28T11:19:48Z |
|
dc.date.issued |
2020 |
|
dc.identifier |
85085676634 |
|
dc.identifier.citation |
journalVolume=21;journalIssueNumber=11;pagination=3825, pages: 18;journalTitle=INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES;journalAbbreviatedTitle=INT J MOL SCI; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/8320 |
|
dc.identifier.uri |
doi:10.3390/ijms21113825 |
|
dc.description.abstract |
(1) Background: Successful treatment of acute kidney injury (AKI)-induced chronic kidney disease (CKD) is unresolved. We aimed to characterize the time-course of changes after contralateral nephrectomy (Nx) in a model of unilateral ischemic AKI-induced CKD with good translational utility. (2) Methods: Severe (30 min) left renal ischemia-reperfusion injury (IRI) or sham operation (S) was performed in male Naval Medical Research Institute (NMRI) mice followed by Nx or S one week later. Expression of proinflammatory, oxidative stress, injury and fibrotic markers was evaluated by RT-qPCR. (3) Results: Upon Nx, the injured kidney hardly functioned for three days, but it gradually regained function until day 14 to 21, as demonstrated by the plasma urea. Functional recovery led to a drastic reduction in inflammatory infiltration by macrophages and by decreases in macrophage chemoattractant protein-1 (MCP-1) and tumor necrosis factor-alpha (TNF-α) mRNA and most injury markers. However, without Nx, a marked upregulation of proinflammatory (TNF-α, IL-6, MCP-1 and complement-3 (C3)); oxidative stress (nuclear factor erythroid 2-related factor 2, NRF2) and fibrosis (collagen-1a1 (Col1a1) and fibronectin-1 (FN1)) genes perpetuated, and the injured kidney became completely fibrotic. Contralateral Nx delayed the development of renal failure up to 20 weeks. (4) Conclusion: Our results suggest that macrophage activation is involved in postischemic renal fibrosis, and it is drastically suppressed by contralateral nephrectomy ameliorating progression. |
|
dc.relation.ispartof |
urn:issn:1661-6596 |
|
dc.title |
Post-Ischemic Renal Fibrosis Progression Is Halted by Delayed Contralateral Nephrectomy : The Involvement of Macrophage Activation |
|
dc.type |
Journal Article |
|
dc.date.updated |
2020-06-15T10:43:31Z |
|
dc.language.rfc3066 |
en |
|
dc.rights.holder |
NULL |
|
dc.identifier.mtmt |
31332871 |
|
dc.identifier.pubmed |
32481551 |
|
dc.contributor.department |
SE/AOK/I/I. Sz. Patológiai és Kísérleti Rákkutató Intézet |
|
dc.contributor.department |
SE/AOK/I/Transzlációs Medicina Intézet |
|
dc.contributor.institution |
Semmelweis Egyetem |
|