dc.contributor.author |
Lányi Árpád |
|
dc.contributor.author |
Baráth Mónika |
|
dc.contributor.author |
Péterfi Zalán |
|
dc.contributor.author |
Bőgel Gábor |
|
dc.contributor.author |
Orient Anna |
|
dc.contributor.author |
Simon Tünde |
|
dc.contributor.author |
Kis-Tóth Katalin |
|
dc.contributor.author |
Sirokmány Gábor |
|
dc.contributor.author |
Rajnavölgyi Éva |
|
dc.contributor.author |
Buday László |
|
dc.contributor.author |
Geiszt Miklós |
|
dc.date.accessioned |
2014-04-02T05:48:26Z |
|
dc.date.available |
2014-04-02T05:48:26Z |
|
dc.date.issued |
2011 |
|
dc.identifier.citation |
pagination=-;
journalVolume=6;
journalIssueNumber=8;
journalTitle=PLOS ONE; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/84 |
|
dc.identifier.uri |
doi:10.1371/journal.pone.0023653 |
|
dc.description.abstract |
Motility of normal and transformed cells within and across tissues requires specialized subcellular structures, e. g. membrane ruffles, lamellipodia and podosomes, which are generated by dynamic rearrangements of the actin cytoskeleton. Because the formation of these sub-cellular structures is complex and relatively poorly understood, we evaluated the role of the adapter protein SH3PXD2B [HOFI, fad49, Tks4], which plays a role in the development of the eye, skeleton and adipose tissue. Surprisingly, we find that SH3PXD2B is requisite for the development of EGF-induced membrane ruffles and lamellipodia, as well as for efficient cellular attachment and spreading of HeLa cells. Furthermore, SH3PXD2B is present in a complex with the non-receptor protein tyrosine kinase Src, phosphorylated by Src, which is consistent with SH3PXD2B accumulating in Src-induced podosomes. Furthermore, SH3PXD2B closely follows the subcellular relocalization of cortactin to Src-induced podosomes, EGF-induced membrane ruffles and lamellipodia. Because SH3PXD2B also forms a complex with the C-terminal region of cortactin, we propose that SH3PXD2B is a scaffold protein that plays a key role in regulating the actin cytoskeleton via Src and cortactin. |
|
dc.relation.ispartof |
urn:issn:1932-6203 |
|
dc.title |
The Homolog of the Five SH3-Domain Protein (HOFI/SH3PXD2B) Regulates Lamellipodia Formation and Cell Spreading |
|
dc.type |
Journal Article |
|
dc.date.updated |
2014-04-01T19:29:51Z |
|
dc.identifier.mtmt |
1721813 |
|
dc.identifier.wos |
000294253500023 |
|
dc.identifier.pubmed |
21886807 |
|
dc.contributor.department |
MTA TTK/Enzimológiai Intézet |
|
dc.contributor.department |
SE/ÁOK/I/Élettani Intézet |
|
dc.contributor.department |
DE/OEC/ÁOK/Immunológiai Intézet |
|
dc.contributor.department |
SE/AOK/I/Orvosi Vegytani, Molekuláris Biológiai és Patobiokémiai Intézet |
|
dc.contributor.department |
SE/AOK/I/ÉI/MTA-SE Lendület Peroxidáz Enzimek Kutatócsoport |
|
dc.contributor.institution |
MTA Természettudományi Kutatóközpont |
|
dc.contributor.institution |
Semmelweis Egyetem |
|
dc.contributor.institution |
Debreceni Egyetem |
|