Show simple item record Visnovitzné Vukman, Krisztina Ferencz, Andrea Fehér, Daniella Juhos, Krisztina Lőrincz, Péter Visnovitz, Tamás Koncz, Anna Pálóczi, Krisztina Seregélyes, Gábor Försönits, András Khamari, Delaram Galinsoga, Alicia Drahos, László Buzás, Edit Irén 2021-09-14T08:57:22Z 2021-09-14T08:57:22Z 2020
dc.identifier 85100721294
dc.identifier.citation journalVolume=10;journalIssueNumber=1;pagination=e12023, pages: 17;journalTitle=JOURNAL OF EXTRACELLULAR VESICLES;journalAbbreviatedTitle=J EXTRACELLULAR VESICL;
dc.identifier.uri doi:10.1002/jev2.12023
dc.description.abstract Abstract Mast cells have been shown to release extracellular vesicles (EVs) in vitro. However, EV-mediated mast cell communication in vivo remains unexplored. Primary mast cells from GFP-transgenic and wild type mice, were grown in the presence or absence of lipopolysaccharide (LPS), and the secreted EVs were separated from the conditioned media. Mast cell-derived EVs were next cultured with LPS-naïve mast cells, and the induction of TNF-α expression was monitored. In addition, primary mast cells were seeded in diffusion chambers that were implanted into the peritoneal cavities of mice. Diffusion chambers enabled the release of GFP+ mast cell-derived EVs in vivo into the peritoneal cavity. Peritoneal lavage cells were assessed for the uptake of GFP+ EVs and for TNF-α production. In vitro, LPS-stimulated mast cell-derived EVs were efficiently taken up by non-stimulated mast cells, and induced TNF-α expression in a TLR4, JNK and P38 MAPK dependent manner. In vivo, using implanted diffusion chambers, we confirmed the release and transmission of mast cell-derived EVs to other mast cells with subsequent induction of TNF-α expression. These data show an EV-mediated spreading of pro-inflammatory response between mast cells, and provide the first in vivo evidence for the biological role of mast cell-derived EVs.
dc.relation.ispartof urn:issn:2001-3078
dc.title An implanted device enables in vivo monitoring of extracellular vesicle-mediated spread of pro-inflammatory mast cell response in mice
dc.type Journal Article 2021-04-04T10:59:53Z
dc.language.rfc3066 en
dc.rights.holder NULL
dc.identifier.mtmt 31808664
dc.identifier.wos 000610415200003
dc.identifier.pubmed 33708356
dc.contributor.department SE/AOK/I/Genetikai, Sejt- és Immunbiológiai Intézet
dc.contributor.department SE/AOK/K/VAROSMAJOR_SZÍVÉRGYÓGY/Kísérletes és Sebészeti Műtéttani Tanszék
dc.contributor.department SE/AOK/I/GSII/MTA-SE Immun-proteogenomikai Extracelluláris Vezikula Kutatócsoport
dc.contributor.institution Semmelweis Egyetem

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