Kivonat:
AIM: To investigate the role of glutathione S-transferase (GST) and
matrix meta lloproteinase-9 (MMP-9) expressions in the development and
progression of reflux esophagitis-Barrett's meta
plasia-dysplasia-adenocarcinoma sequence in the esophagus.
METHODS: GST and MMP-9 expressions were analyzed in 51
paraffin-embedded tissue samples by immunohistochemistry including
patients with reflux esophagitis (n = 7), Barrett's metaplasia (n =
14), Barrett and esophagitis (n = 8), Barrett and dysplasia (n = 7),
esophageal adenocarcinoma (n = 8) and a control group without any
histological changes (n = 7). Immunostaining was determined
semiquantitatively. Statistical analysis with one-way ANOVA, LSD test
and correlation analysis were performed. P value of < 0.05 was
considered significant.
RESULTS: GST expression was significantly higher while MMP-9 expression
was significantly lower in control group compared to Barrett's
metaplasia and the other groups. No major changes were observed between
Barrett, esophagitis, and Barrett and concomitant esophagitis. Barrett
and concomitant dysplasia, and adenocardnoma revealed a significant
lower expression of GST and higher levels of MMP-9 compared to all
other groups. Adenocarcinoma showed almost no expression of GST and
significantly higher levels of MMP-9 than Barrett and concomitant
dysplasia. Alterations of GST and MMP-9 were inversely correlated (r
0.82).
CONCLUSION: Decreased GST and increased expression of MMP-9 in
Barrett's meta plasia-clysplasiaadenocarcinoma sequence as compared to
normal tissue suggest their association with esophageal tumorigenesis.
Loss of GST and gain of MMP-9 in Barrett with dysplasia compared to
non-clysplastic metaplasia indicate that these alterations may be early
events in carcinogenesis. Quantification of these parameters in
Barrett's esophagus might be useful to identify patients at higher risk
for progression to cancer. (c) 2007 The WJG Press. All rights reserved.
