Kivonat:
Previously, it has been suggested that hypoxia-inducible factor
(HIF) 1 signaling may play determinative role in the maintenance
of the barrier function of the intestinal epithelium in
inflammatory bowel disease. Our aim was to depict the alteration
of HIF-1α and related genes in celiac disease (CD) where the
importance of the barrier function is well known. Duodenal
biopsy specimens were collected from 16 children with untreated
CD, 9 children with treated CD and 10 controls. HIF-1α, trefoil
factor 1 (TFF1), ecto-5-prime nucleotidase (CD73), and multi
drug resistance gene 1 (MDR1) mRNA and HIF-1α protein expression
were determined by real-time PCR and Western blot, respectively.
Localization of HIF-1α was determined by immunofluorescent
staining. We found increased HIF-1α and TFF1 mRNA and HIF-1α
protein expression in the duodenal mucosa of children with
untreated CD compared with controls or children with treated CD
(p < 0.05). In untreated CD children, HIF-1α staining was
present in cytoplasmic and nuclear region of the villous
enterocytes. In treated CD mRNA expression of CD73 and MDR1 were
increased compared with controls (p < 0.01 and 0.05,
respectively). Our results of increased mucosal HIF-1α
expression in CD children suggest the contribution of this
signaling pathway in the pathomechanism of CD. Copyright © 2010
International Pediatric Research Foundation, Inc.