dc.contributor.author |
Vannay, Ádám |
|
dc.contributor.author |
Sziksz, Erna |
|
dc.contributor.author |
Prókai, Ágnes |
|
dc.contributor.author |
Veres, Gábor |
|
dc.contributor.author |
Molnár, Kriszta |
|
dc.contributor.author |
Nagy-Szakál, Dorottya |
|
dc.contributor.author |
Ónody, Anna |
|
dc.contributor.author |
Korponay-Szabó, Ilma Rita |
|
dc.contributor.author |
Szabó, András |
|
dc.contributor.author |
Tulassay, Tivadar |
|
dc.contributor.author |
Arató, András |
|
dc.contributor.author |
Szebeni, Beáta |
|
dc.date.accessioned |
2018-10-10T13:08:57Z |
|
dc.date.available |
2018-10-10T13:08:57Z |
|
dc.date.issued |
2010 |
|
dc.identifier |
77955145363 |
|
dc.identifier.citation |
pagination=118-122;
journalVolume=68;
journalIssueNumber=2;
journalTitle=PEDIATRIC RESEARCH; |
|
dc.identifier.uri |
http://repo.lib.semmelweis.hu//handle/123456789/6146 |
|
dc.identifier.uri |
doi:10.1203/PDR.0b013e3181e5bc96 |
|
dc.description.abstract |
Previously, it has been suggested that hypoxia-inducible factor
(HIF) 1 signaling may play determinative role in the maintenance
of the barrier function of the intestinal epithelium in
inflammatory bowel disease. Our aim was to depict the alteration
of HIF-1α and related genes in celiac disease (CD) where the
importance of the barrier function is well known. Duodenal
biopsy specimens were collected from 16 children with untreated
CD, 9 children with treated CD and 10 controls. HIF-1α, trefoil
factor 1 (TFF1), ecto-5-prime nucleotidase (CD73), and multi
drug resistance gene 1 (MDR1) mRNA and HIF-1α protein expression
were determined by real-time PCR and Western blot, respectively.
Localization of HIF-1α was determined by immunofluorescent
staining. We found increased HIF-1α and TFF1 mRNA and HIF-1α
protein expression in the duodenal mucosa of children with
untreated CD compared with controls or children with treated CD
(p < 0.05). In untreated CD children, HIF-1α staining was
present in cytoplasmic and nuclear region of the villous
enterocytes. In treated CD mRNA expression of CD73 and MDR1 were
increased compared with controls (p < 0.01 and 0.05,
respectively). Our results of increased mucosal HIF-1α
expression in CD children suggest the contribution of this
signaling pathway in the pathomechanism of CD. Copyright © 2010
International Pediatric Research Foundation, Inc. |
|
dc.relation.ispartof |
urn:issn:0031-3998 |
|
dc.title |
Increased expression of hypoxia-inducible factor 1α in coeliac disease |
|
dc.type |
Journal Article |
|
dc.date.updated |
2018-08-24T08:00:43Z |
|
dc.language.rfc3066 |
en |
|
dc.identifier.mtmt |
1384930 |
|
dc.identifier.wos |
000280211900006 |
|
dc.identifier.pubmed |
20453713 |
|
dc.contributor.department |
SE/AOK/K/ISZGYK/MTA-SE Gyermekgyógyászati és Nephrológiai Kutatócsoport |
|
dc.contributor.department |
SE/AOK/K/I. Sz. Gyermekgyógyászati Klinika |
|
dc.contributor.institution |
Semmelweis Egyetem |
|